GDVII and DA isolates of Theiler's virus: proteins attached to the 5' end of the RNA are bound covalently to the same nucleotides
- 1 March 1983
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 45 (3) , 1150-4
- https://doi.org/10.1128/jvi.45.3.1150-1154.1983
Abstract
GDVII and DA picornaviruses, which represent two biologically distinct subgroups of Theiler's murine encephalomyelitis viruses, were analyzed to detect the presence of a RNA-linked protein, VPg. It was found that the single-stranded RNA genomes isolated from the virions of both viruses contain a protein of approximately 7,000 daltons, which is covalently linked to the 5' end of the RNA. Like in other picornaviruses (e.g., poliovirus and encephalomyocarditis virus), the terminal nucleotide adjacent to the protein was found to be pUp. The possibility that differently charged VPg species exist is also mentioned.Keywords
This publication has 14 references indexed in Scilit:
- Replication of Theiler's murine encephalomyelitis viruses in BHK21 cells: An electron microscopic studyVirology, 1980
- Protein is linked to the 5' end of poliovirus RNA by a phosphodiester linkage to tyrosineJournal of Biological Chemistry, 1978
- The genome-linked protein of picornaviruses IV. Difference in the VPg's of encephalomyocarditis virus and poliovirus as evidence that the genome-linked proteins are virus-codedVirology, 1978
- Protein covalently linked to foot-and-mouth disease virus RNANature, 1977
- The location of the polio genome protein in viral RNAs and its implication for RNA synthesisNature, 1977
- Covalent linkage of a protein to a defined nucleotide sequence at the 5'-terminus of virion and replicative intermediate RNAs of poliovirus.Proceedings of the National Academy of Sciences, 1977
- A protein covalently linked to poliovirus genome RNA.Proceedings of the National Academy of Sciences, 1977
- Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.Proceedings of the National Academy of Sciences, 1976
- Theiler's virus infection in mice: an unusual biphasic disease process leading to demyelination.1975
- Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4Nature, 1970