Gram Scale Purification and Preparation of Rabbit Liver Zinc Metallothionein
- 1 June 1992
- journal article
- research article
- Published by Taylor & Francis in Preparative Biochemistry
- Vol. 22 (2) , 151-164
- https://doi.org/10.1080/10826069208021365
Abstract
A simplified procedure for purifying gram quantities of rabbit liver metallothionein (MT) using gel filtration and anion exchange chromatography is presented. The MT purification made use of anion exchange batch elution chromatography which greatly shortened the procedure. Quantitation techniques for use with crude and purified MT are discussed. This paper also describes the preparation of large amounts of ZnMT from Cd,ZnMT.Keywords
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