Isoelectric Focusing and Reverse Immunoblotting of Autoantibodies Against High Molecular Weight Antigens
- 1 January 1986
- journal article
- research article
- Published by Taylor & Francis in Immunological Investigations
- Vol. 15 (2) , 113-122
- https://doi.org/10.3109/08820138609094137
Abstract
A method for the clonal analysis of antibodies against high M.Wt. antigens is described. It involves isoelectric focusing, electrophoretic transfer of the focused antibodies to a nitrocellulose membrane and detection of the membrane-bound antibodies by overlay with radiolabelled antigen (reverse immunoblotting). The application of this technique to the clonal analysis of autoantibodies against thyroglobulin and DNA is described.This publication has 17 references indexed in Scilit:
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