Restricted subpopulations of DNA antibodies in kidneys of mice with systemic lupus

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Abstract
Sera from MRL/1, BXSB, and NZB/NZW mice, which develop IgG antibodies to DNA and glomerular deposits of DNA‐antiDNA immune complexes, were studied by isoelectric focusing. A large array of IgG antibodies with isoelectric points ranging from pH 5.5‐9.0 were found to bind double‐stranded DNA. Antibodies with isoelectric points from 8.0‐8.5 were significantly more frequent than antibodies focusing in all other pH ranges. In contrast, glomerular eluates from MRL/1 and NZB/NZW mice contained a restricted number of DNA‐binding bands, all of which focused at pH 8.0‐9.0. Anti‐DNA with isoelectric points from pH 8.0‐9.0 may be more pathogenic for the kidney than other subpopulations.