Production of infectious poliovirus from cloned cDNA is dramatically increased by SV40 transcription and replication signals
Open Access
- 1 January 1984
- journal article
- Published by Oxford University Press (OUP) in Nucleic Acids Research
- Vol. 12 (12) , 5123-5141
- https://doi.org/10.1093/nar/12.12.5123
Abstract
Sub-genomic cDNA clones representing the entire genomic RNA of poliovirus Type 1 (Mahoney) have been isolated in E. coli. Construction of a complete cDNA copy of the poliovirus genome in the EcoRI site of plasm id vector pBR325 from these clones is described. Introduction of plasmid DNA containing the complete cDNA copy of polio RNA into cultured primate cells by transfection produces infectious poliovirus. The virus produced by such a transfection appears to be identical to wild type poliovirus. Isolation of a polio recombinant plasmid containing SV40 transcription and replication signals is also described. Transfection of COS-1 cells with this plasmid yields greater than 1,600 plaque-forming units (PFU) per ug of input DNA.Keywords
This publication has 39 references indexed in Scilit:
- Construction and characterization of new cloning vehicle. II. A multipurpose cloning systemGene, 1977
- Characteristics of a Human Cell Line Transformed by DNA from Human Adenovirus Type 5Journal of General Virology, 1977
- Synthesis of extensive, possibly complete, DNA copies of poliovirus RNA in high yields and at high specific activities.Proceedings of the National Academy of Sciences, 1976
- Function of simian virus 40 gene A in transforming infectionJournal of Virology, 1975
- Isolation of a Viral Polypeptide Associated with Poliovirus RNA PolymeraseProceedings of the National Academy of Sciences, 1974
- Requirement of 3′-Terminal Poly(adenylic acid) for the Infectivity of Poliovirus RNAProceedings of the National Academy of Sciences, 1974
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973
- A simple method of preparing large amounts of ΦX174 RF I supercoiled DNABiochimica et Biophysica Acta (BBA) - Nucleic Acids and Protein Synthesis, 1973
- Polyadenylic Acid at the 3′-Terminus of Poliovirus RNAProceedings of the National Academy of Sciences, 1972
- Calcium-dependent bacteriophage DNA infectionJournal of Molecular Biology, 1970