Trivaline ‘catalyzes’ 5′‐pdGTT oligomerization in solution

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Abstract
We have found that the 5′‐pdGTT molecules at a concentration of 10−4 M are oligomerized in solution in the presence of 10−4 M tripeptide ‐ (L‐Val)3‐NH‐NH‐DNS‐CF3COOH and the condensation reagents (carbodiimide and imidazole). Oligonucleotides not less than 12 bases long were formed in the yield which was over 15%. It is known that in the absence of peptide 10−2 M mono‐ or dinucleotides are required. Thus trivaline can be considered as one of the simplest enzymes. This oligomerization seems to be an essential way for the synthesis of long enough oligonucleotides of the random GC‐sequence, which could be used at the earliest steps of evolution.

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