Gas Chromatographic Determination of Hydrolyzed Sugars in Commercial Gums

Abstract
A modified GC method was utilized for the detection and quantitative determination of sugars derived from commercial gum, Agar, Carageenan, Xanthan, Gum Ghatti, Gum Arabic, Emulgum, Gum Tragacanth, and Gum Karaya. The polysaccharides were cleaved by acid hydrolysis, derivatized with “STOX” hydroxylamine to form oxime with “TMSI” or “HMDS+TFAA” for silylation. The separation and quantitation was performed by gas chromatography, using phenyl‐BD‐glucopyranoside as the internal standard for quantitative analysis. Sugar molar ratios (percent) for all commercial gums tested were similar to literature values.

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