Strand Invasion of Extended, Mixed-Sequence B-DNA by γPNAs
- 7 August 2009
- journal article
- research article
- Published by American Chemical Society (ACS) in Journal of the American Chemical Society
- Vol. 131 (34) , 12088-12090
- https://doi.org/10.1021/ja900228j
Abstract
In this communication, we show that peptide nucleic acids (PNAs) with lengths of 15−20 nucleotides, when preorganized into a right-handed helix, can invade mixed-sequence double-helical B-form DNA (B-DNA). Strand invasion occurs in a highly sequence-specific manner through direct Watson−Crick base pairing. Unlike the previously developed double-duplex invasion strategy, which requires simultaneous binding of two strands of pseudocomplementary PNAs to DNA, only a single strand of γPNA is required for invasion in this case, and no nucleobase substitution is needed.Keywords
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