Effects of vasopressin on phosphoinositides and prostaglandin production in cultured mesangial cells

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Abstract
The effects of vasopressin on phospholipid metabolism in [rat] mesangial cells and the temporal relationship of these changes to prostaglandin production were studied. The changes included: increased breakdown of phosphatidylinositol (PI), increased breakdown of diphosphoinositide (PI-P) and triphosphoinositide (PI-P2); an increase in the mass of diglyceride and phosphatidic acid; increased synthesis, first of PI-P and PI-P2, then phosphatidic acid and finally, PI; and increased water-soluble inositol phosphates. Vasopressin treatment resulted in a significant increase (90%) in the mass of phosphatidic acid and a smaller (13%) decline in the mass of PI. Changes in diglyceride were seen following 45 s of treatment with vasopressin. Alterations of phosphoinositide metabolism were seen as early as 45 s and continued for up to 5 h following hormone exposure. By contrast, prostaglandin production declined after 30 min. These observations on vasopressin-stimulated metabolism in cultured mesangial cells may provide a basis for an understanding of the functional changes that follow hormone exposure.