THE PRECISION OF UNBIASED ESTIMATES OF NUMERICAL DENSITY OF ENDOTHELIAL CELLS IN DONOR CORNEAS

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Abstract
The precision of estimates of central corneal endothelial density was studied in 16 human corneas stained by alizarin red and trypan blue. Estimates based on central counts and estimates based on peripheral counts were considered separately. The numerical density was estimated employing an unbiased sampling technique. From central counts estimates with an error of less than 5% could be obtained. From peripheral counts a maximum precision of mean .+-. 12.2% could be obtained. The theroretical maximum precision was calculated by application of a variance component model. The precision of estimates was calculated for 1, 2 and 4 test areas of different sizes. Economy of sampling was evaluated by comparison of the actual precision to the theoretical maximum precision of estimates.

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