STUDIES ON THE MECHANISM OF OVULATION

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Abstract
The incorporation of S35 into follicles of the rabbit ovary was investigated by the Pelc stripping film technique. The greater part of the tracer dose of S35 was excreted and most of the retained portion was in the tissue sulpho-mucopolysaccharides. The labelled sulphate first accumulated in the granulosa cells and from there it reached the ovarian follicular fluid. The metachromatic intercellular substance of the granulosa-cell layer did not take up radiosulphur which indicated that it contained a non-sulphuric mucopolysaccharide. The granulosa cells gave off sulphuric mucopolysaccharide to the follicular fluid at the time of antrum formation. After coitus or gonadotropin injection which increased the follicle size, there was no further release of sulpho-mucopolysaccharide from the granulosa cells. No radioactive sulphur was found in the fresh corpora lutea (tertiary follicular fluid or in the ovocyte).