Structure of Haloacetate-Catabolic IncP-1β Plasmid pUO1 and Genetic Mobility of Its Residing Haloacetate-Catabolic Transposon
Open Access
- 15 November 2003
- journal article
- research article
- Published by American Society for Microbiology in Journal of Bacteriology
- Vol. 185 (22) , 6741-6745
- https://doi.org/10.1128/jb.185.22.6741-6745.2003
Abstract
The self-transmissible plasmid pUO1 from Delftia acidovorans strain B carries two haloacetate-catabolic transposons, Tn Had1 and Tn Had2 , and the mer genes for resistance to mercury. The complete 67,066-bp sequence of pUO1 revealed that the mer genes were also carried by two Tn 402 /Tn 5053 -like transposons, Tn 4671 and Tn 4672 , and that the pUO1 backbone regions shared 99% identity to those of the archetype IncP-1β plasmid R751. Comparison of pUO1 with three other IncP-1β plasmids illustrated the importance of transposon insertion in the diversity and evolution of this group of plasmids. Mutational analysis of the four outermost residues in the inverted repeats (IRs) of Tn Had2 , a Tn 21 -related transposon, revealed a crucial role of the second residue of its IRs in transposition.Keywords
This publication has 16 references indexed in Scilit:
- The Movement of Tn3‐Like Elements: Transposition and Cointegrate ResolutionPublished by American Society for Microbiology ,2007
- The 79,370-bp conjugative plasmid pB4 consists of an IncP-1β backbone loaded with a chromate resistance transposon, the strA-strB streptomycin resistance gene pair, the oxacillinase gene bla NPS-1, and a tripartite antibiotic efflux system of the resistance-nodulation-division familyMolecular Genetics and Genomics, 2003
- Complete Nucleotide Sequence and Organization of the Atrazine Catabolic Plasmid pADP-1 from Pseudomonas sp . Strain ADPJournal of Bacteriology, 2001
- Mobile catabolic genes in bacteriaJournal of Bioscience and Bioengineering, 1999
- Complete sequence of the IncPβ plasmid R751: implications for evolution and organisation of the IncP backboneJournal of Molecular Biology, 1998
- Four genes, two ends, and a res region are involved in transposition of Tn5053: a paradigm for a novel family of transposons carrying either a mer operon or an integronMolecular Microbiology, 1995
- A functional analysis of the inverted repeat of the γδ transposable elementJournal of Molecular Biology, 1995
- Chlorobenzoate catabolic transposon Tn5271 is a composite class I element with flanking class II insertion sequences.Proceedings of the National Academy of Sciences, 1991
- Functional analysis of the two domains in the terminal inverted repeat sequence required for transposition of Tn3Gene, 1991
- Physical and genetic analysis of the Inc-W group plasmids R388, Sa, and R7KPlasmid, 1982