Template boundary definition in Tetrahymena telomerase
Open Access
- 15 February 2002
- journal article
- Published by Cold Spring Harbor Laboratory in Genes & Development
- Vol. 16 (4) , 415-420
- https://doi.org/10.1101/gad.962602
Abstract
Telomerase elongates chromosome ends by addition of telomeric DNA repeats. The telomerase ribonucleoprotein can copy only a short template sequence within the telomerase RNA subunit. Here, we identify a region of telomerase RNA that is necessary for both correct 5′ template boundary definition and high affinity telomerase reverse transcriptase (TERT) interaction. We also demonstrate that TERT mutants in the RNA binding domain compromise both 5′ boundary definition and RNA binding. Our results indicate that sequence-specific interaction of a telomerase RNA element with the TERT RNA binding domain, not the active site motifs, defines the template boundary.Keywords
This publication has 18 references indexed in Scilit:
- Expression of mutant telomerase in immortal telomerase-negative human cells results in cell cycle deregulation, nuclear and chromosomal abnormalities and rapid loss of viabilityOncogene, 2001
- RNA Binding Domain of Telomerase Reverse TranscriptaseMolecular and Cellular Biology, 2001
- TELOMERES AND THEIR CONTROLAnnual Review of Genetics, 2000
- Reversing Time: Origin of TelomeraseCell, 1998
- d-AMINO ACIDS IN ANIMAL PEPTIDESAnnual Review of Biochemistry, 1997
- Block in Anaphase Chromosome Separation Caused by a Telomerase Template MutationScience, 1997
- Boundary elements of the Tetrahymena telomerase RNA template and alignment domains.Genes & Development, 1995
- Runaway telomere elongation caused by telomerase RNA gene mutationsNature, 1995
- Ciliate telomerase RNA structural featuresNucleic Acids Research, 1995
- A conserved secondary structure for telomerase RNACell, 1991