The Asparagine-Linked Sugar Chains of the Glycoproteins in Calf Thymocyte Plasma Membrane

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Abstract
The neutral oligosaccharide fraction obtained from the hydrazinolysate of the plasma membrane glycoproteins of calf thymocytes was shown to be a mixture of twelve oligosaccharides. Oligosaccharides A, B, and C, which released galactose on treatment with jack bean β-galacto-sidase but not with diplococcal β-galactosidase, were shown to have the structures Galβ1→3Galβ1→4GlcNAcβ1→4(Galβ1→3Galβ1→4GlcNAcβ1→2)Manα1→6(3)[Galβ1→3Galβ1→4GlcNAcβ1→6(Galβ1→3Galβ1→4GlcNAcβ1→2)Manα1→3(6)]Manβ1→3(6)]Manβ1→4GlcNAcβ→4(Fucα1→6)GlcNAc, Galβ1→3Galβ1→4GlcNAcβ1;→4(Galβ1→3Galβ1→4GlcNAcβ1→2)Manα1→6(3)[Galβ1→3Galβ1→4GlcNAcβ1→4(Fucα1→6)GlcNAc, and Glaβ1→3Galβ1→4GlcNAcβ1→2Manα1→6(Galβ1→3Galβ1→4GlcNAcβ1→2Manα1→3)Manβ1→5GlcNAcβ1→4(Fucα1→6)GlcNAc, respectively. Oligosaccharides D and E, which released galactose on treatment with either jack bean β-galactosidase or diplococcal β-galactosidase, were shown to have the structures Galβ1→4GlcNAcβ2Manα1→(Galβ1→4GlcNAcβ1→2Manα1→3)Manβ1→4GlcNAcβ1→4(Fucα1→)GlcNAc and Galβ1→4GlcNAcβ1→2Manα1→(GlcNAcβ1→2Manβ1→3)Manβ1→4GlcNAcβ1→5(Fucα1→)GlcNAc, respectively. Five oligosaccharides (F1, G, H1, I, and J) were shown to have typical high-mannose type structures (Manαl→)n. Manβ1 → GIcNAcβ1→GlcNAc, with n-values of 8, 7, 6, 5, and 4, respectively. The structures of the remaining two oligosaccharides F2, and H2 were elucidated as GlcNAcβ1 → 2Manαl → (GlcNAcβl → 2Manαl → 3)Manβl → 4GlcNAcβl→ 4(Fucαl→)-GlcNAc and Manαl →(Manαl→)Manβ1→(GlcNAcβ→Manαl→)Manβ1→GlcNAcβ1 →GlcNAc, respectively.

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