CYTOKINE-ACTIVATED HUMAN ENDOTHELIAL-CELLS SYNTHESIZE AND SECRETE A MONOCYTE CHEMOATTRACTANT, MCP-1/JE

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  • 1 June 1990
    • journal article
    • research article
    • Vol. 136  (6) , 1229-1233
Abstract
We have demonstrated inducible expression of the mRNA encoding the monocyte chemoattractant MCP-1, the human homolog of the JE gene, in endothelial cells within 3 hours of treatment with IL-1.beta. and tumor necrosis factor. IFN-.gamma. also induced expression of this mRNA after 24 hours, but to a lesser extent. MCP-1/JE protein steadily accumulated in the medium of endotheial cells during a 48-hour exposure to IL-1.beta.. Medium conditioned by IL-1.beta.-treated endothelial cells contained monocyte chemoattractant activity that was immunoadsorbed by anti-MCP-1 antibodies. These results suggest that endothelial cells secrete a monocyte chemoattractant, MCP-1/JE, in response to inflammatory mediators, and thus may contribute to the accumulation of monocyte at sites of inflammation.