Electrofusion ofPenicilliumprotoplasts after dielectrophoresis
- 10 January 1987
- journal article
- research article
- Published by Wiley in Journal of Basic Microbiology
- Vol. 27 (6) , 341-344
- https://doi.org/10.1002/jobm.3620270614
Abstract
The protoplast fusion of auxotrophicPenicilliummutats after dielectrophoretic cell contact and rectangular field pulses (4,2 kV/cm, 25 μs) was microscopically observed in a microchamber consisting of two vapour‐plated chromium electrodes on a microslide (gap between the electrodes of about 150 μm). The conditions for protoplasting ofPenicilliumand for electrofusion are described. Isolation of viable fusion products was realized.Keywords
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