A Comparative Study of the Nutritional Requirements of Salmonella typhosa, Salmonella pullorum and Salmonella gallinarum

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Abstract
A comparative study was made of the vitamin, amino acid and glucose requirements of S. pullorum, S. gallinarum and S. typhosa, with the chief aim of establishing new means of differentiating those closely related organisms, particularly the 2 fowl salmonellas. S. gallinarum (22 strains) was the only ono of the three which required a growth factor in the synthetic medium, namely, vitamin B. S. typhosa alone (9 strains) failed to develop in the absence of any particular amino acid; it demanded tryptophane. S. pullorum was the most variable in its response to different amino acids. Most of the strains of S. pullorum refused to grow-in the absence of glucose; some were indifferent, and a few were near the borderline. Glucose was definitely beneficial to all strains. This was in contrast to the deportment of S. galliuarum, which revealed no need for this carbohydrate. 8 of the S. gallinarum strains required added CO2for growth in the synthetic medium. None of S. pullorum and S. typhosa showed this need.