Decreasing the number of 68-base-pair tandem repeats in the BK virus transcriptional control region reduces plaque size and enhances transforming capacity
- 1 September 1985
- journal article
- research article
- Published by American Society for Microbiology in Journal of Virology
- Vol. 55 (3) , 823-825
- https://doi.org/10.1128/jvi.55.3.823-825.1985
Abstract
Wild-type BK virus, which has three 68-base-pair (bp) elements tandemly repeated in its transcriptional control region, forms clear, large plaques in human embryonic kidney cell cultures but rarely transforms hamster or rat cells. From this BK virus DNA, deletion mutants with fewer than three 68-bp elements were constructed and characterized. The mutant DNA with two 68-bp elements formed small, turbid plaques in human cell cultures and transformed rat 3Y1 cells inefficiently. The mutant DNA with one 68-bp element formed minute, turbid plaques in human cell cultures and transformed rat cells efficiently.This publication has 13 references indexed in Scilit:
- Selective extraction of polyoma DNA from infected mouse cell culturesPublished by Elsevier ,2004
- Expression of viral early functions in rat 3Y1 cells infected with human papovavirus BKJournal of Virology, 1984
- BK Viral Enhancer Element and a Human Cellular HomologScience, 1983
- The adenovirus type 5 E1A transcriptional control region contains a duplicated enhancer elementCell, 1983
- Multiple Point Mutations Affecting the Simian Virus 40 EnhancerScience, 1983
- Definition of the simian virus 40 early promoter region and demonstration of a host range bias in the enhancement effect of the simian virus 40 72-base-pair repeat.Proceedings of the National Academy of Sciences, 1983
- Tumorigenicity of human bk papovavirus plaque isolates, wild-type and plaque morphology mutant, in hamstersInternational Journal of Cancer, 1982
- BK Virus DNA: Complete Nucleotide Sequence of a Human Tumor VirusScience, 1979
- A new technique for the assay of infectivity of human adenovirus 5 DNAVirology, 1973
- Enchancement of the infectivity of simian virus 40 deoxyribonucleic acid with diethylaminoethyl-dextran.1968