Spontaneous51Cr release by isolated rat hepatocytes: An indicator of membrane damage
- 1 August 1978
- journal article
- research article
- Published by Springer Nature in In Vitro Cellular & Developmental Biology - Plant
- Vol. 14 (8) , 707-714
- https://doi.org/10.1007/bf02616167
Abstract
Radiochromium uptake and release by isolated rat hepatocytes in suspension was monitored under continuous-labeling conditions. Cell protein remained unchanged during the absorption phase, whereas the release of51Cr correlated well with the loss of cell viability and release of cytoplasmic protein. The results suggest that under equilibrium conditions,51Cr release represents an efflux of label from damaged or dying preparations and not an elution of radioisotope from intact cells.Keywords
This publication has 32 references indexed in Scilit:
- Plasma protein synthesis by isolated rat hepatocytes.The Journal of cell biology, 1977
- (51Cr) sodium chromate incorporation into the soluble protein fraction of the human erythrocyte: Binding not associated with the hemoglobin monomeric subunitBiochemical and Biophysical Research Communications, 1976
- A long-term51chromium assay forin vitro cell-mediated tumor immunity. Correlation with simultaneously performed microplate assaysInternational Journal of Cancer, 1975
- Synthesis and secretion of fibrinogen and albumin by isolated rat hepatocytesBiochemical and Biophysical Research Communications, 1974
- Secretion of serum albumin by enzymatically isolated rat liver cellsFEBS Letters, 1974
- CYTOSTATIC ELIMINATION OF SYNGENEIC RAT TUMOR CELLS IN VITRO BY NONSPECIFICALLY ACTIVATED MACROPHAGESThe Journal of Experimental Medicine, 1973
- Intact endoplasmic reticulum and albumin synthesis in rat liver cell suspensionsExperimental Cell Research, 1971
- The Proportion of the Incorporation of Leucine into Albumin to that into Total Protein in Rat Liver and Hepatoma Morris 5123 TC*European Journal of Biochemistry, 1969
- DISTRIBUTION OF ALLOGENEIC 51Cr-LABELLED LYMPH NODE CELLS IN MICETransplantation, 1966
- HUMAN LYMPHOCYTE TYPING WITH ISOLOGOUS ANTISERA; TECHNICAL CONSIDERATIONS AND A PRELIMINARY STUDY OF THE CYTOTOXIC REACTION SYSTEMTransplantation, 1965