Egg plant lipoxygenase: Isolation and partial characterization
- 1 July 1972
- Vol. 7 (7) , 467-473
- https://doi.org/10.1007/bf02533163
Abstract
Egg plant lipoxygenase EC 1.13.1.13 when purified on Ecteola cellulose was resolved into two active fractions with most of the activity in the first fraction (A). This fraction when further purified on Sephadex G200‐120 had 20 times the specific activity of the crude material. It proved to be a single substance by electrophoresis and immunological technique. The pH optimum was 6.5. Its activity was specific for thecis,cis‐1,4 pentadiene structure. There was no inhibition by cyanide, azide, EDTA or fluoride. Nordihydroguaretic acid, on the other hand, exhibited strong inhibition at 3 × 10−3M concentration. The specific antibody caused 50% inhibition.Keywords
This publication has 25 references indexed in Scilit:
- Intracellular distribution of lipoxygenase-like activity of alfalfa leavesPhytochemistry, 1972
- LIPOXYGENASE CONTENT AND ANTIOXIDANT ACTIVITY OF SOME FRUITS AND VEGETABLESJournal of Food Science, 1971
- Lipoxidase activity of leavesPhytochemistry, 1970
- Enzymic oxidation of carotene and linoleate by alfalfa: Extraction and separation of active reactionsPhytochemistry, 1969
- Carotene bleaching activity of tomato extractsJournal of the Science of Food and Agriculture, 1968
- Chemical Coupling of Proteins to AgaroseNature, 1967
- Evaluation of Lipid Oxidation in Plant TissuesJournal of Food Science, 1966
- DISC ELECTROPHORESIS – II METHOD AND APPLICATION TO HUMAN SERUM PROTEINS*Annals of the New York Academy of Sciences, 1964
- Reactions of Cytochrome-c with Methyl Linoleate HydroperoxideNature, 1961
- Alfalfa Lipoxidase.Plant Physiology, 1956