A SNaPshot assay for the rapid and simple detection of four common hotspot codon mutations in the PIK3CA gene
Open Access
- 1 January 2009
- journal article
- Published by Springer Nature in BMC Research Notes
- Vol. 2 (1) , 66
- https://doi.org/10.1186/1756-0500-2-66
Abstract
Activating mutations in the PIK3CA gene have been identified in a variety of human malignancies and are commonly detected in hotspot codons located in the helical and kinase domains in exons 9 and 20. Existing methodologies for the detection of PIK3CA mutations are time-consuming and/or expensive. In the present study we describe the first application of a PIK3CA SNaPshot assay to the screening of frequent mutations in these exons.Keywords
This publication has 20 references indexed in Scilit:
- Somatic mutation and gain of copy number of PIK3CA in human breast cancerBreast Cancer Research, 2005
- PIK3CA mutations in glioblastoma multiformeActa Neuropathologica, 2005
- Frequent Mutation of the PIK3CA Gene in Ovarian and Breast CancersClinical Cancer Research, 2005
- PIK3CA Mutations Correlate with Hormone Receptors, Node Metastasis, and ERBB2, and Are Mutually Exclusive with PTEN Loss in Human Breast CarcinomaCancer Research, 2005
- Mutations of PIK3CAin gastric adenocarcinomaBMC Cancer, 2005
- PIK3CA mutations in advanced ovarian carcinomasHuman Mutation, 2005
- PIK3CA gene is frequently mutated in breast carcinomas and hepatocellular carcinomasOncogene, 2004
- Mutation of the PIK3CA Gene in Ovarian and Breast CancerCancer Research, 2004
- The phosphatidylinositol 3-Kinase–AKT pathway in human cancerNature Reviews Cancer, 2002
- The phosphatidylinositol 3'-kinase p85alpha gene is an oncogene in human ovarian and colon tumors.2001