Paper-chromatographic separation of chlorophylls and carotenoids from marine algae

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Abstract
Two-dimensional paper chromatography with propan-1-ol-light petroleum and chloroform-light petroleum mixtures separated the chloroplast pigments of marine algae belonging to the classes Chlorophyceae, Bacillariophyceae, Dinophyceae and Chrysophyceae. The pigments separated included chlorophylls a, b and c, their pheophytins, the carotenes, the xanthophylls lutein, violaxanthin, neoxanthin, fucoxanthin and isomers, peridinin, esterified astaxanthin and a number of minor xanthophyll components. The concentration of the accessory chlorophylls b and c was of the same order of magnitude as that of chlorophyll a. Fucoxanthin and peridinin, and their isomers, constituted 80-90% of the total carotenoids in the organisms which contained these pigments.

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