Bilirubin and the van den Bergh reaction

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Abstract
Bilirubin, obtained from gallstones by crystallization from chloroform soln., was insoluble in serum and gave a delayed van den Bergh reaction when suspended in serum. The amorphous pigment, obtained by treating the filtrate and washings from the above crystals with light petroleum, is soluble in serum and gave a much more prompt reaction. The monosodium salt and the disodium salt of bilirubin gave delayed reactions. A direct-reacting pigment was obtained by precipitation with various phosphates, caustic alkalis, and ammonium carbonate. A pigment, isolated from bile after addition of HC1, was soluble in serum and gave a prompt reaction. A pigment isolated from bile without acidification gave a prompt reaction. The author concluded that bilirubin may exist in two forms, each giving rise to a separate series of salts.

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