In vitro activity of Triclisia patens and some bisbenzylisoquinoline alkaloids against eishmania donovani and Trypanosoma brucei brucei

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Abstract
In the search for antiprotozoal compounds from natural sources, Triclisia patens displayed activity against L. donovani promastigotes (IC50 = 1.5 μg/mL) and T. b. brucei blood stream trypomastigote forms (IC50 = 31.25 μg/mL). In addition, a total of 20 bisbenzylisoquinoline alkaloids were screened for antileishmanial and antitrypanosomal activity in vitro. Fangchinoline (IC50 = 0.39 μM) was found to be as active as the standard pentamidine against Leishmania donovani promastigotes. Phaeanthine was three-fold more active (IC50 = 2.41 μM; 1.5 μg/mL) than the standard drug Pentostam against L. donovani amastigotes, but at this concentration was toxic to murine macrophages. In contrast, cocsoline (IC50 = 12.3 μM; 6.76 μg/mL) was as active as Pentostam, and was not toxic to macrophages at this concentration. Thalisopidine showed the strongest activity (IC50 = 1.14 μM) against Trypanosoma brucei brucei blood stream form trypomastigotes, but was less active than pentamidine. Copyright © 2002 John Wiley & Sons, Ltd.