Isolation of two different pools of pituitary prolactin
- 1 September 1984
- journal article
- research article
- Published by Oxford University Press (OUP) in Acta Endocrinologica
- Vol. 107 (1) , 25-30
- https://doi.org/10.1530/acta.0.1070025
Abstract
Two pools of prolactin (Prl) were isolated from the pituitary gland. One was readily soluble after homogenization and the other sedimented with secretory granules. In ovariectomized-estrogen treated rats the latter represents the major Prl fraction of pituitary and can be recovered as immunoreactive Prl after extraction in 2.5 M urea. By correlation of EM, chromatography and radioimmunoassay, evidence is gathered that the soluble fraction is constituted by monomeric (little) Prl and that secretory granules contain Prl in a polymerized form (big).This publication has 8 references indexed in Scilit:
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