Callus and embryoid formation from protoplasts of Helianthus annuus
- 1 October 1988
- journal article
- research article
- Published by Springer Nature in Plant Cell Reports
- Vol. 7 (6) , 437-440
- https://doi.org/10.1007/bf00269532
Abstract
Sunflower hypocotyl protoplasts have been isolated and cultured. Optimum plating density for cell division and colony formation was in the range of 5 to 7×104 cells/mi in an agarose medium supplemented with BAP (1 mg/l) and NAA (1 mg/l). Plating efficiency was 60% after 21 days of culture. In the resultant culture a mixed population of calli and embryoids was observed. Thirty seven percent of the cell clusters exhibited a developmental pattern similar to an embryoid. Many stages of embryogenesis were observed in the same cultures.Keywords
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