An Interferon-Induced 16-kD Protein Is Present in the Membranes of Interferon-Sensitive but Not in Interferon-Resistant Mouse Cells

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Abstract
Human interferon-α (IFN-α) or IFN-β has been shown to induce a 17-kD membrane protein in human cells which when eluted from SDS gels inhibited the multiplication of cells of different human cell lines. We show herein that mouse IFN-α/β induces a 16-kD membrane protein in L1210 and Friend erythroleukemia cells sensitive to IFN-α/β, (but not in the derived IFN-α/β-resistant cell lines) as well in primary and monolayer cultures of mouse embryonic fibroblasts and adult mouse hepatocytes, and in suspensions of spleen cells. In addition, IFN-α/β enhanced the expression of an 11-kD membrane protein which could be shown by immunoprecipitation to be β2-microglobulin. Anticell proliferation activity was not recovered from the 16-kD fraction of the SDS gels.