Seminal Fluid Androgen Binding Protein

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Abstract
The presence of an androgen binding protein (ABP) in the ejaculate was investigated. Seminal fluid was obtained from 10 fertile subjects. Sperm counts ranged from 20 .times. 106/cc to 80 .times. 106/cc. Each sample was preincubated with [3H]-dihydrotestosterone (DHT) and examined by polyacrylamide gel electrophoresis (PAGE). After preincubation of the samples with 3H-DHT, the samples were mixed with Concanavalin-A bound to Sepharose-D (C-S) and the supernate separated and counted. Saturation analysis of the samples using dextran-coated charcoal for separation was performed and Scatchard analysis was used to determine the concentration of ABP present. PAGE demonstrated ABP in 7 of 10 samples studied. The bound counts of DHT were removed by C-S. The concentrations of ABP ranged from 0.132 to 0.468 nm ABP/dl semen. Apparently ABP in seminal fluid is similar to that from the Sertoli cell.