Enzymatic Synthesis of Glutamine in Higher Plants

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Abstract
An enzyme system was isolated from a number of plant tissues which catalyzes the synthesis of the amide bond of glutamine by a direct condensation of glutamic acid and ammonia. The synthesis requires the presence of adenosine triphosphate and Mg ions. No necessity for additional co-factors was detected. The synthetic activity is associated with the mitochondrial fraction of cellular homo-genates.