Gamma ray-induced loss of expression of HLA and glyoxalase I alleles in lymphoblastoid cells.

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Abstract
Gamma rays from a Ce source were used to generate human lymphoblastoid cell line variants that had lost expression of all major histocompatibility complex antigens coded for by a single haplotype. The cell line was heterozygous at the glyoxalase I locus and had the HLA haplotyes HLA-A1, B8, DRw3 and HLA-A2, B5 DRw1. Anti-HLA-B8 antiserum was used for selection in a population of cells that had been irradiated with 300 R .gamma.-rays. The incidence of B8-loss variants was 4.1 .times. 10-5 on day 5 after irradiation. Analysis of variants showed that expressions of HLA and GLO alleles trans to B8 were retained. Expression of additional cis-linked HLA and GLO gene products was lost in 12 of 17 variants. Variants that had lost expression of HLA-B8; HLA-B8, A1; HLA-B8, A1, DRw3; or HLA-B8, A1, DRw3 and the cis-linked glyoxalase I allele were obtained. Karyotype analysis was preformed on 8 variants that had lost expression of 2 or more cis-linked alleles. Three variants had 2 normal appearing 6th chromosomes, 4 variants had a deletion that included the region coding for HLA genes on the short arm of one chromosome 6 and 1 variant had an inversion or translocation involving the short arm of one chromosome 6.