Phorbol ester stimulates the synthesis of sphingomyelin in NIH 3T3 cells A diminished response in cells transformed with human A‐raf carrying retrovirus

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Abstract
The tumor promoter 12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) stimulated the synthesis of sphingomyelin (CerPCho) from a [14C]choline‐labelled phosphatidylcholine (PtdCho) pool in NIH 3T3 cells. Maximal stimulation (68%) of CerP‐Cho synthesis, accompanied by an increase (38%) in its cellular content, required only 2 nM TPA. Higher concentrations of TPA (2–100 nM) had progressively less effect on CerPCho synthesis which correlated with increased hydrolysis of precursor PtdCho. In cells transformed with human or mouse A‐raf carrying retroviruses TPA‐stimulated PtdCho hydrolysis, but not CerPCho synthesis, suggesting independent regulation of these processes by the TPA‐stimulated signal transduction system.

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