INFECTION OF TOBACCO MESOPHYLL PROTOPLASTS BY TOBACCO MOSAIC VIRUS
- 1 November 1969
- journal article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 64 (3) , 843-848
- https://doi.org/10.1073/pnas.64.3.843
Abstract
It was proved that the protoplasts prepared from mesophyll of Nicotiana tabacum are infected by tobacco mosaic virus. The infection occurred when purified tobacco mosaic virus particles were added to a protoplast suspension in the presence of poly-L-ornithine. The virus multiplied in these protoplasts to a level of 10(6) virus particles per infected protoplast during 24 hours of incubation. The efficiency of infection was remarkably high, exceeding that by mechanical inoculation of tobacco leaves.Keywords
This publication has 5 references indexed in Scilit:
- Fluorescent antibody staining of tobacco mosaic virus antigen in tobacco mesophyll protoplastsVirology, 1969
- Uptake of Protein by Mammalian Cells: An Underdeveloped AreaScience, 1968
- Amino acid sequence of a common japanese strain of tobacco mosaic virusJournal of Molecular Biology, 1968
- Protoplasts: Preparation from Higher PlantsScience, 1966
- Relation between incorporation of 2-thiouracil into tobacco mosaic virus nucleic acid and virus inhibitionVirology, 1962