Serological Relationships Among Membrane Proteins of Strains ofXanthomonas campestrispv.Translucens

Publisher Website
Google Scholar
Abstract
The serological relationships among 26 strains of Xanthomonas campstris pv. translucens from wheat, barley, rye, triticale, wild rice, and weeds were determined with antisera to membrane proteins. One or two major bands of precipitin were observed in Ouchterlony agar double-diffusion tests. The band nearest the antigen well (hereafter referred to as the M1 band) was consistently present, whereas the other band (M2) was not. Based on the M1 band and cross-absorption tests, 25 of the 26 strains tested were grouped into two serovars and two subserovars. One strain was untypable. Of the 44 other bacteria tested, only X. c. pv. begoniae strain B-935 could not be distinguished from X. c. pv. translucens. In constrast to the high specificity observed in Ouchterlony tests, immunofluorescence (IF) tests resulted in low specificity. Several other anthomonads, pseudomonads, and erwiniae could not be differentiated from X. c. pv. translucens based on IF tests. Although strains of X. c. pv. translucens were more virulent on their hosts of origin and showed variable pathogenicity reactions on different hosts, there was no correlation between host of origin and serovar.