Molecular cloning and sequence analysis of human preprocathepsin C
- 7 August 1995
- journal article
- Published by Wiley in FEBS Letters
- Vol. 369 (2-3) , 326-330
- https://doi.org/10.1016/0014-5793(95)00777-7
Abstract
A cDNA clone (C1) coding for human preprocathepsin C was isolated from a human ileum cDNA library using a rat kidney‐derived RT‐PCR probe and its complete nucleotide sequence determined. The full‐length 1857 bp sequence codes for a protein of 463 amino acid residues with a calculated molecular mass of 51848 Da. Comparison of the deduced amino acid sequence with that of rat preprocathepsin C indicates an 87.5% identity. A multiple alignment of the deduced cathepsin C sequence of 233 residues which, by analogy to other cysteine proteinases, corresponds to the mature protein, confirms that human cathepsin C belongs to the papain superfamily.Keywords
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