Limited size of the fragile X site shown by fluorescence in situ hybridization
- 15 April 1992
- journal article
- Published by Wiley in American Journal of Medical Genetics
- Vol. 43 (1-2) , 187-191
- https://doi.org/10.1002/ajmg.1320430131
Abstract
Cosmids, isolated from a 475 kb YAC that spans the fragile X region, and the YAC itself, were used for fluorescence in situ hybridization (FISH) on metaphase chromosomes from fragile X patients. Cosmid 22.3, containing most of the hybrid translocation breakpoints, shows in situ hybridization signals distal and proximal from the fragile X site. We propose that the size of the fragile site is limited to 20 kb.Keywords
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