Studies of Human Insulin from Nondiabetic and Diabetic Pancreas
- 1 October 1967
- journal article
- research article
- Published by American Diabetes Association in Diabetes
- Vol. 16 (10) , 687-694
- https://doi.org/10.2337/diab.16.10.687
Abstract
Insulin has been isolated from single human pancreases from diabetic and nondiabetic sources. Purification was accomplished primarily by gel filtration. The average yield of insulin from the nondiabetic pancreas was 8.6 mg/100 gm of tissue, and from the diabetic pancreas 2.9 mg/100 gm of tissue, and from the diabetic pancreas 2.9 mg/100 gm. The amino acid composition of nondiabetic insulin was in agreement with the known structure of human insulin. The insulin isolated from the diabetic pancreas had the same amino acid composition as normal human insulin in all cases but 1. Based upon the amino acid analysis, it is possible that a different amino acid sequence occurs in the insulin from this diabetic source.This publication has 8 references indexed in Scilit:
- Recent Advances Relative to Diabetes MellitusAnnals of Internal Medicine, 1965
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- Isolation of insulin from pancreatic extracts using carboxymethyl and diethylaminoethyl cellulosesBiochimica et Biophysica Acta (BBA) - General Subjects, 1964
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