BIOSYNTHESIS OF THE TWO CHAINS OF INSULIN
- 1 April 1965
- journal article
- research article
- Published by Proceedings of the National Academy of Sciences in Proceedings of the National Academy of Sciences
- Vol. 53 (4) , 853-859
- https://doi.org/10.1073/pnas.53.4.853
Abstract
Islet tissue minces from the fish Lophius piscatorius have been incubated with proline-C14 for 8 hr. and with proline-H3 for 15 or 30 min. at 17[degree]C. After incubation, the minces were combined, insulin was extracted, purified, and separated into 3 peptides, each containing 1 proline residue. The 3 proline residues were isolated and their H3/C14 ratios determined. From these radioactivity of values and from knowledge of the positions of proline in the chains, evidence was obtained for the conclusion that the 2 constituent chains of insulin are synthesized separately.Keywords
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