Benzodiazepine Receptors on Primary Cultures of Mouse Astrocytes

Abstract
Benzodiazepines bind to glial membranes on a single type of site, with a high affinity (KD= 5 × 10−9 M) on about 100 fmol of sites per mg protein. The number of binding sites is increased when the membranes are treated with Triton X‐100. Antiepileptic drugs such as clonazepam and phenobarbital and hypnotic drugs such as Ro‐11‐3128 and Ro‐11‐6896 are able in pharmacological concentrations to displace [3H]flunitrazepam from its glial binding sites.