SECRETION OF INTERLEUKIN-1 BY ACUTE MYELOBLASTIC-LEUKEMIA CELLS-INVITRO INDUCES ENDOTHELIAL-CELLS TO SECRETE COLONY STIMULATING FACTORS
- 1 October 1987
- journal article
- research article
- Vol. 70 (4) , 1218-1221
Abstract
The interaction of acute myeloblastic leukemia (AML) cells with stromal cells was investigated by adding AML-conditioned media to cultures of human endothelial cells. This conditioned media contained factors that induced expression of both the granulocyte macrophage colony stimulating factor (GM-CSF) and granulocyte CSF (G-CSF) genes and release of colony stimulating activity from endothelial cells. The conditioned media contained interleukin-1 (IL-1) bioactivity and the endothelial cell stimulatory activity was partially neutralized by anti-IL-1 antiserum. Constitutive expression of the IL-1-beta gene was detected in ten of 17 AML cases analyzed. These results suggest that the unregulated secretion of IL-1 by AML cells can induce stromal cells in vitro to overproduce CSFs. This could contribute to the unrestricted growth of AML cells.This publication has 11 references indexed in Scilit:
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