IGG ANTIHAPTEN ANTIBODY SECRETION INVITRO COMMENCES AFTER EXTENSIVE PRECURSOR PROLIFERATION

  • 1 January 1977
    • journal article
    • research article
    • Vol. 33  (4) , 485-490
Abstract
Experiments reported here show that intermediate cells that arise in primed mice after boosting require further cycles of division in culture before maturation into Ig[immunoglobulin]G antibody secreting cells. Killing of dividing cells between days 1-4 in culture, by exposure to BUdR[bromodeoxyuridine]-UV irradiation ablated the high IgG response observed on day 5 in control cultures. After T[thymus-derived]-cell removal and replacement by a soluble factor (TFR) similar results were obtained. B [bone marrow-derived] cell division over an extended period occurs prior to the appearance of IgG secreting cells. Autoradiography of plaques from cultures briefly exposed to [3H]-thymidine before harvest showed that some antibody secreting cells were synthesizing DNA at the time of assay.