A polycation causes migration of negatively charged phospholipids from the inner to outer leaflet of the liposomal membrane
- 28 February 1994
- journal article
- Published by Wiley in FEBS Letters
- Vol. 340 (1-2) , 121-123
- https://doi.org/10.1016/0014-5793(94)80185-1
Abstract
Aggregation of the negatively charged liposomes caused by the addition of the linear polycation, poly‐N‐ethyl‐4‐vinylpyridine bromide, was studied. At the point of maximal size and zero electrophoretic mobility of aggregates, the concentration of positive charges brought in by the adsorbed polycation was found to be equal to the total concentration of negative charges both on the outer and inner surface of the lipid bilayer. Since polycation saturation of the liposomal negative charges was found to occur without disruption of the membrane, it was concluded that the polycation induced migration of negatively charged phospholipid molecules from the inner to outer leaflet of the bilayer.Keywords
This publication has 16 references indexed in Scilit:
- Design of target-specific polymer conjugates and complexes for biomedical applicationMakromolekulare Chemie. Macromolecular Symposia, 1990
- Quasi-elastic and electrophoretic light scattering studies of the reorganization of dioleoylphosphatidylcholine vesicle membranes by poly(2-ethylacrylic acid)Macromolecules, 1989
- Stability of polymer-complexed phospholipid vesiclesJournal of Colloid and Interface Science, 1988
- Low pH induced membrane fusion of lipid vesicles containing proton-sensitive polymerBiochemistry, 1987
- The induction of large unilamellar vesicle fusion by cationic polypeptides: the effects of mannitol, size, charge density and hydrophobicity of the cationic polypeptidesBiochimica et Biophysica Acta (BBA) - Biomembranes, 1986
- Polyhistidine mediates an acid-dependent fusion of negatively charged liposomesBiochemistry, 1984
- Soluble interpolymeric complexes as a new class of synthetic polyelectrolytesPure and Applied Chemistry, 1984
- Investigation of the inside-outside distribution, intermembrane exchange and transbilayer movement of phospholipids in sonicated vesicles by shift reagent NMRBiochimica et Biophysica Acta (BBA) - Biomembranes, 1980
- Comparison of properties of an oxime-bound partially quaternized poly-4-vinylpyridine and a monomer analogous oximeEuropean Polymer Journal, 1974
- Structure of macromolecular aggregates. II. Construction of model membranes from phospholipids and polypeptidesBiochemistry, 1970