DESIGN OF MULTIDYE SYSTEMS FOR FRET-BASED APPLICATIONS
- 31 March 2001
- journal article
- Published by Taylor & Francis in Nucleosides, Nucleotides and Nucleic Acids
- Vol. 20 (4-7) , 369-374
- https://doi.org/10.1081/ncn-100002308
Abstract
A new solid phase approach, based on orthogonal protective group strategy utilizing Fmoc and DMTr groups, was used to assemble linear polymeric chains with pending groups at desired locations. A compound synthesized using four different fluorophores with consequently overlapping absorption and emission spectra (pyrene, perylene, fluorescein and TAMRA) was shown to fluoresce at 570 nm when excited at 330 nm, demonstrating sequential energy transfer across four chromophores.Keywords
This publication has 7 references indexed in Scilit:
- Tritylisation of pyrene, perylene and coronene: a new family of switchable fluorescent labelsTetrahedron Letters, 2000
- Sequential multistep energy transfer: enhancement of efficiency of long-range fluorescence resonance energy transferChemical Communications, 1999
- Signaling Recognition Events with Fluorescent Sensors and SwitchesChemical Reviews, 1997
- Steric factors influencing hybridisation of nucleic acids to oligonucleotide arraysNucleic Acids Research, 1997
- Building large porphyrin arrays: pentamers and nonamersChemical Communications, 1996
- Oligodeoxyribonucleotides containing 1, 3-propanediol as nucleoside substituteNucleic Acids Research, 1987
- Zwischenmolekulare Energiewanderung und FluoreszenzAnnalen der Physik, 1948