A New Staining Technique For Microfilariae

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Abstract
A rapid whole mount staining method is described to identify and differentiate microfilariae [human blood parasite] without elaborate processing. A single solution combining Hoyer''s mounting medium and hematoxylin stain facilitates light microscopic examination of nuclei and sheaths of microfilariae. The new technique stains microfilariae adequately in 3-7 min at 60.degree. C making the method preferable to conventional methods that may take as long as 45-60 min. Lantern heat may be used to heat slides in rural areas with good results.