ATP‐dependent Ca2+ transport in vesicles isolated from the bile canalicular region of the hepatocyte plasma membrane
Open Access
- 1 February 1985
- journal article
- research article
- Published by Wiley in European Journal of Biochemistry
- Vol. 147 (1) , 1-7
- https://doi.org/10.1111/j.1432-1033.1985.tb08710.x
Abstract
Three plasma membrane subfractions have been isolated and characterized from rat liver cells. The high affinity Ca2+-stimulated ATPase is highly enriched in the bile canalicular subfraction. Taking into account cross-contamination by the blood sinusoidal and lateral membranes it is suggested that the high-affinity Ca2+ -ATPase is located exclusively in this fraction. The high-affinity Ca2+-ATPase is coupled to Ca2+ transport, is calmodulin-insensitive, sensitive to vanadate under appropriate experimental conditions and is strongly inhibited by La3+. In the presence of Ca2+ and ATP the ATPase forms a phosphorylated intermediate of molecular mass about 200 kDa.This publication has 25 references indexed in Scilit:
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