Enhancing effect of centrifugation on isolation of influenza virus from clinical specimens
- 1 July 1990
- journal article
- research article
- Published by American Society for Microbiology in Journal of Clinical Microbiology
- Vol. 28 (7) , 1669-1670
- https://doi.org/10.1128/jcm.28.7.1669-1670.1990
Abstract
The use of centrifugation (700 x g, 60 min) in a plaque assay markedly increased (mean, 2.9-fold) the infectivity of all 42 influenza virus strains tested, compared with no centrifugation. Of 13 influenza virus strains isolated from 390 clinical specimens, 9 (69%) were efficiently isolated by the centrifugation assay compared with conventional culture methods. The centrifugation assay may be useful for isolating the influenza virus from clinical specimens.This publication has 16 references indexed in Scilit:
- Rapid diagnosis of influenza infection of NP antigen using an immunocapture ELISA testJournal of Virological Methods, 1989
- Comparison of three techniques for detection of respiratory viruses in nasopharyngeal aspirates from children with lower acute respiratory infectionsJournal of Medical Virology, 1989
- Rapid 24-well plate centrifugation assay for detection of influenza A virus in clinical specimensJournal of Virological Methods, 1989
- One-incubation time-resolved fluoroimmunoassay based on monoclonal antibodies in detection of influenza A and B viruses directly in clinicalJournal of Virological Methods, 1989
- Characterization and evaluation of monoclonal antibodies developed for typing influenza A and influenza B virusesJournal of Clinical Microbiology, 1986
- Rapid detection of cytomegalovirus in MRC-5 cells inoculated with urine specimens by using low-speed centrifugation and monoclonal antibody to an early antigenJournal of Clinical Microbiology, 1984
- Mechanisms of herpes simplex virus infectivity enhanced by ultracentrifugal inoculationInfection and Immunity, 1980
- Ultracentrifugal inoculation of herpes simplex virusInfection and Immunity, 1978
- Permanent canine kidney (MDCK) cells for isolation and plaque assay of influenza B virusesMedical Microbiology and Immunology, 1975
- Plaque assay and primary isolation of influenza a viruses in an established line of canine kidney cells (MDCK) in the presence of trypsinMedical Microbiology and Immunology, 1975