Two temperature-sensitive mutants of Saccharomyces cerevisiae with altered expression of mating-type functions.

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Abstract
Two mutants of S. cerevisiae were isolated from normal haploid MAT.alpha. strains and characterized as having temperature-sensitive, pleiotropic phenotypes for functions associated with mating. At the permissive temperature, 23.degree. C, they were found to behave as normal MAT.alpha. haploids with respect to mating efficiency, sporulation in diploids formed with MATa strains, secretion of .alpha.-factor, and failure to secrete the MATa-specific products, a-factor and Barrier. At higher temperatures they declined in mating and sporulation efficiency and to express the a-specific functions. Genetic analysis established that 1 of these mutants, PE34, carries a temperature-sensitive allele of the MAT.alpha.2 gene and that the other, PD7, carries a temperature-sensitive allele of the TUP1 gene.