Bimane fluorescent labels: labeling of normal human red cells under physiological conditions.

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Abstract
The bimane fluorescent labels, monobromobimane, dibromobimane and monobromotrimethylammoniobimane are derivatives of syn-9,10-dioxabimane:1,5-diazabicyclo[3.3.0]octa-3,6-diene-2,8-dione. They efficiently labeled Hb (reactive thiol groups), membrane proteins and glutathione of normal human red cells [RBC] under physiological conditions. Monobromobimane and dibromobimane were effective on intact cells while RBC membranes may be impermeable to positively charged monobromotrimethylammoniobimane, the latter being effective only on lysed cells. These bimane labels provide a class of labeling agents that may have wide applicability in biological materials.