Absence of Immunoreactive Cytokines in Supernatants of Individual Preimplantation Human Embryos
- 10 September 1993
- journal article
- Published by Wiley in American Journal of Reproductive Immunology
- Vol. 30 (2-3) , 105-107
- https://doi.org/10.1111/j.1600-0897.1993.tb00610.x
Abstract
PROBLEM: The purpose of this study was to determine if it was possible to use commercially available enzyme‐linked immunosorbent assay (ELISA) kits to quantitate specific cytokine production from individual embryos. METHODS: We examined the supernatants of individual two to six cell preimplantation human embryos using commerically available kits for IL‐1 alpha, IL‐1 beta, IL‐6, and LIF. RESULTS: Cytokines were undetectable in all media samples analyzed. CONCLUSION: While it is possible that individual preimplantation embryos may produce cytokines, we believe these concentrations are below the detectable limits of the commercial assays used in this study.Keywords
This publication has 4 references indexed in Scilit:
- Cytokine involvement in oocytes and early embryosFertility and Sterility, 1991
- Prediction of successful embryo implantation by measuring interleukin-1-alpha and immunosuppressive factor(s) in preimplantation embryo culture fluidFertility and Sterility, 1991
- The genes for leukemia inhibitory factor and interleukin-6 are expressed in mouse blastocysts prior to the onset of hemopoiesis.Molecular and Cellular Biology, 1990
- Immunosuppressive Factor (or Factors) Produced by Human Embryos in VitroNew England Journal of Medicine, 1986