An Inhibitor of Interferon Action: I. Physical Association of the Inhibitor with Interferon-gamma

Abstract
Previously, an inhibitor of interferon action has been identified in mouse lymphokine preparations. The inhibitor was produced following IFN-γ production and thus may be involved in regulation of IFN-γ action. We now show that native inhibitor activity as isolated from crude and partially purified IFN-γ preparations migrates along with interferon activity on molecular sieving gels. IFN-γ which comigrates with inhibitor migrates at a slightly higher molecular weight than IFN-γ free of inhibitor. This inhibitor activity correlates with the decreased antiviral activity of crude IFN-γ produced late in the course of mitogen stimulation. This is apparent for both plaque reduction and yield reduction assays for interferon. This association of IFN-γ with its inhibitor suggests a mechanism for the local regulation of IFN-γ activity.

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