Enterococcal Aggregation Substance and Binding Substance Are Not Major Contributors to Urinary Tract Colonization by Enterococcus faecalis in a Mouse Model of Ascending Unobstructed Urinary Tract Infection
Open Access
- 1 April 2004
- journal article
- research article
- Published by American Society for Microbiology in Infection and Immunity
- Vol. 72 (4) , 2445-2448
- https://doi.org/10.1128/iai.72.4.2445-2448.2004
Abstract
Isogenic Enterococcus faecalis strains that differ in their expression of aggregation substance (AS) and its cognate receptor, enterococcal binding substance (EBS), were compared for urovirulence in mice. Strain OG1SSp/pCF500 (inducible AS + , constitutive EBS + ) failed to outcompete isogenic derivative INY3000 (AS − EBS − ) in the urine, bladders, or kidneys of mice harvested at 48 h postinoculation. Neither mouse nor human urine induced AS expression by OG1SSp/pCF500. Recombinant strain OG1SSp/pINY1801 (constitutive AS + , EBS + ) exhibited plasmid segregation that was as extensive in vivo as in vitro. These data suggest that AS and EBS do not contribute to upper or lower urinary tract colonization by E. faecalis and that growth in urine does not induce AS expression by strains carrying plasmids in the pCF10 family.Keywords
This publication has 28 references indexed in Scilit:
- IroN Functions as a Siderophore Receptor and Is a Urovirulence Factor in an Extraintestinal Pathogenic Isolate of Escherichia coliInfection and Immunity, 2002
- The Enterococcus faecalis fsrB Gene, a Key Component of the fsr Quorum-Sensing System, Is Associated with Virulence in the Rabbit Endophthalmitis ModelInfection and Immunity, 2002
- Genogrouping and Incidence of Virulence Factors ofEnterococcus faecalisin Liver Transplant Patients Differ from Blood Culture and Fecal IsolatesThe Journal of Infectious Diseases, 2002
- Evidence that the Enterococcal Polysaccharide Antigen Gene ( epa ) Cluster Is Widespread in Enterococcus faecalis and Influences Resistance to Phagocytic Killing of E. faecalisInfection and Immunity, 2002
- Role of Enterococcus faecalis Surface Protein Esp in the Pathogenesis of Ascending Urinary Tract InfectionInfection and Immunity, 2001
- Aggregation Substance Promotes Adherence, Phagocytosis, and Intracellular Survival ofEnterococcus faecaliswithin Human Macrophages and Suppresses Respiratory BurstInfection and Immunity, 2000
- Analysis of a Gene Cluster ofEnterococcus faecalisInvolved in Polysaccharide BiosynthesisInfection and Immunity, 2000
- Defining Inoculation Conditions for the Mouse Model of Ascending Urinary Tract Infection that Avoid Immediate Vesicoureteral Reflux yet Produce Renal and Bladder InfectionThe Journal of Infectious Diseases, 1996
- Incidence of Hemolysin, Gelatinase, and Aggregation Substance among Enterococci Isolated from Patients with Endocarditis and Other Infections and from Feces of Hospitalized and Community-Based PersonsThe Journal of Infectious Diseases, 1995
- A Plasmid-Encoded Surface Protein On Enterococcus Faecalis Augments Its Internalization By Cultured Intestinal Epithelial CellsThe Journal of Infectious Diseases, 1994